Over the years, there has been increasing demand of sensors for clinical diagnosis, environmental, and industrial applications with strong requirements concerning selectivity, sensitivity and speed. In this manuscript readers can able to find out the cost-effective, sensitive, selective and easy to operate technique for ultimate use in the detection of biomolecules ranging from micro- to macromolecular size at trace level, which is fabricated for common people. In this book author try to incorporate both the fundamentals and wide application of target-specific materials, which is popularly called as molecularly imprinted polymers (MIP). In this hardcover, readers can find out a detail overview of MIP-based electrochemical sensors fabrication for the detection and enantio-differentiation of D- and L-enantiomers of biomolecules acts as effective biomarkers for some very common diseases viz., folic acid for chrohn’s disease, epilepsy, anemia; tryptophan for schizophrenia and other neural related diseases; thyroxine for clinical treatment of thyroid & maintaining its normal growth; insulin for diabetes mellitus.