Plant Chemical Genomics: Methods and
Protocols , expert researchers in the field detail
many of the techniques used for identification of
bioactive compounds from a large selection of fields in
plant biology including plant pathogenesis, immune
responses, small RNA processing, endomembrane
trafficking, lipids, plant hormone signaling and cell
wall. The presentation of these and other examples as
well as synthetic chemistry, computation and target
identification provides a comprehensive overview of the
practical aspects of chemical biology that are possible
in plant systems. Small molecules and natural products
that produce a phenotype in plants have been known for
decades. The agrichemical industry has utilized small
molecules based on known natural molecules such as
auxins, cytokinins, abscissic acid and other growth
regulators. In recent years, the screening and
characterization of novel small molecules has enhanced
our understanding of protein function, metabolic,
signaling and endomembrane pathways, and their
interactions in basic plant research. Written in the
highly successful Methods in Molecular Biology
series format, chapters include introductions to
their respective topics, lists of the necessary
materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on
troubleshooting and avoiding known pitfalls.
|
|