Description Like its predecessor Lab Ref, Volume 1, this book is a handy benchtop source of recipes and information needed for common laboratory protocols. Lab Ref, Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, Lab Ref, Volume 2 also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software. Contents: Introduction: SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS: Isolation of Total DNA; Isolation of Total RNA; Selection of Poly(A) RNA from Total; Isolation and Amplification of RNA; from Single Cells and Small Amounts of Tissue; Hybridization Analysis; Preparation of Nucleic Acid (Target) for Array Hybridization Analysis; Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis; Other Quantitative Methods; SECTION 2: RNAi: RNAi in C. elegans; RNAi in D. melanogaster; RNAi in Plants; RNAi in Mammalians; RNAi in Mouse Oocytes and Early Embryos; SECTION 3: IMAGING: Preparation of Embryos for Live Cell Imaging; Imaging Fixed Tissue; Preparation of Mice for Imaging; Intracellular Imaging; General Information for Imaging; SECTION 4: PROTEIN ANALYSIS/PROTEOMICS: Basic Methods in Protein Analysis for Proteomics; Extraction of Proteins; Advanced Protocols in Identifying Protein Protein Interactions for Proteomics; SECTION 5: ARABIDOPSIS: Microscopy; Genetic Manipulation; Protein Extraction; Nuclear Extraction; Histochemistry of GUS Fusion; SECTION 6: MURINE: In Vitro Culturing of Embryonic Stem Cells; Genotyping; Immunohistochemistry of Whole-mount Embryos; Other Buffers and Solutions; SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES: SECTION 8: USEFUL FACTS AND TABLES: A. Useful Information and Reference Tables; B. Nomenclature Guide; C. Useful World Wide Web Sites; Appendix; Index. Note from the Publisher: You may be interested in the following title: Labe Ref volume 1