Hepatitis viruses clinical and laboratory diagnosis overview with simple approach for undergraduate and early post graduate in biomedical science field. It discusses viruses, genotypes, simple laboratory techniques for detection, molecular techniques. Complete discussion of viruses hepatitis A, B, C, D and E was carried out in the text. New techniqes in molecular biology and serology have provided evidence of blood-transmitted viruses as hepatitis F (HFV) and hepatitis G (HGV). The HGV is found worldwide and in United States is more prevalent than HCV.HGV is closely related to HCV and is sometimes called GB virus C (GBV-C). Apparently, however, it is so well adapted to its human hosts that it causes no significant disease condition. About 5% of cases of chronic liver disease can not be attributed to any known hepatitis in known series A through E