Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. Botanically, garlic belongs to the genus Allium, family Alliaceae. The aim of this investigation is to induction of genetic variation by gamma rays radiation in garlic, the use of genetic variability in irradiated plants as a source of genetic material for genetic improvement by individual selection, a trial for vegetative propagation in the irradiated material by tissue culture technique and evaluation of plants produced from irradiated cloves at field conditions.