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Contemporary approaches to the synthesis of
chemically modified biomacromolecules (proteins, nucleic
acids, lipids, and carbohydrates) not only require
efficient means to control conjugation and the specific
site of attachment of the conjugated moiety but also the
effective use of recent developments in the fields of
pharmaceutical chemistry, biomolecular/polymer
engineering, and nanobiotechnology. In this second
edition of Bioconjugation Protocols: Strategies
and Methods, expert researchers update the classic
methods and introduce valuable new approaches that go
beyond basic conjugation techniques to include elements
from advanced organic synthesis, molecular biology,
surface biotechnology, materials science, and
nanobioscience/engineering. These readily
reproducible methods cover the preparation of
biomolecular conjugates using a variety of labeling
techniques and semisynthetic approaches.
Additional chapters address the biofunctionalization of
surface structures, including organic/inorganic thin
films, as well as various types of nanostructures
(magnetic nanoparticles, quantum dots, carbon nanotubes,
and silicon nanowire devices). All the protocols
follow the successful Methods in Molecular
BiologyTM series format, each one
offering step-by-step laboratory instructions, an
introduction outlining the principle behind the
technique, lists of the necessary equipment and
reagents, and tips on troubleshooting and avoiding known
pitfalls.
Cutting-edge and highly
practical, Bioconjugation Protocols: Strategies and
Methods, Second Edition offers both novice and
experienced researchers access to the broad array of
techniques needed to carry out the semisynthesis of
functional biomolecular reagents and/or the
biofunctionalization of surfaces and structures of
unique interest for a wide variety of applications,
ranging from novel biomedical diagnostics to powerful
new therapeutics to advanced biomaterials.
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